RACK-1 directs dynactin-dependent RAB-11 endosomal recycling during mitosis in Caenorhabditis elegans.

ABSTRACT: Membrane trafficking pathways are necessary for the addition and removal of membrane during cytokinesis. In animal cells, recycling endosomes act as a major source of the additional membranes during furrow progression and abscission. However, the mechanisms and factors that regulate recycling endosomes during the cell cycle remain poorly understood. Here, we show that the Caenorhabditis elegans Receptor of Activated C Kinase 1 (RACK-1) is required for cytokinesis, germline membrane organization, and the recruitment of RAB-11-labeled recycling endosomes to the pericentrosomal region and spindle. RACK-1 is also required for proper chromosome separation and astral microtubule length. RACK-1 localizes to the centrosomes, kinetochores, the midbody, and nuclear envelopes during the cell cycle. W

SUBMITTER: Ai E 

PROVIDER: S-EPMC2655251 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature