Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP.

ABSTRACT: The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.

SUBMITTER: Biteen JS 

PROVIDER: S-EPMC2655310 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature