Molecular diversity of a North Carolina wastewater treatment plant as revealed by pyrosequencing.

ABSTRACT: We report the results of pyrosequencing of DNA collected from the activated sludge basin of a wastewater treatment plant in Charlotte, NC. Using the 454-FLX technology, we generated 378,601 sequences with an average read length of 250.4 bp. Running the 454 assembly algorithm over our sequences yielded very poor assembly, with only 0.3% of our sequences participating in assembly of significant contigs. Of the 117 contigs greater than 500 bp long that were assembled, the most common annotations were to transposases and hypothetical proteins. Comparing our sequences to known microbial genomes showed nonspecific recruitment, indicating that previously described taxa are only distantly related to the most abundant microbes in this treatment plant. A comparison of proteins generated by translati

SUBMITTER: Sanapareddy N 

PROVIDER: S-EPMC2655459 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature