An efficient platform for genetic selection and screening of gene switches in Escherichia coli.

ABSTRACT: Engineered gene switches and circuits that can sense various biochemical and physical signals, perform computation, and produce predictable outputs are expected to greatly advance our ability to program complex cellular behaviors. However, rational design of gene switches and circuits that function in living cells is challenging due to the complex intracellular milieu. Consequently, most successful designs of gene switches and circuits have relied, to some extent, on high-throughput screening and/or selection from combinatorial libraries of gene switch and circuit variants. In this study, we describe a generic and efficient platform for selection and screening of gene switches and circuits in Escherichia coli from large libraries. The single-gene dual selection marker tetA was translationa

SUBMITTER: Muranaka N 

PROVIDER: S-EPMC2655682 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature