Regulator of G-protein signaling 14 (RGS14) is a selective H-Ras effector.

ABSTRACT:

Background

Regulator of G-protein signaling (RGS) proteins have been well-described as accelerators of Galpha-mediated GTP hydrolysis ("GTPase-accelerating proteins" or GAPs). However, RGS proteins with complex domain architectures are now known to regulate much more than Galpha GTPase activity. RGS14 contains tandem Ras-binding domains that have been reported to bind to Rap- but not Ras GTPases in vitro, leading to the suggestion that RGS14 is a Rap-specific effector. However, more recent data from mammals and Drosophila imply that, in vivo, RGS14 may instead be an effector of Ras.

Methodology/principal findings

Full-length and truncated forms of purified RGS14 protein were found to bind indiscriminately in vitro to both Rap- and Ras-family GTPases, consistent with prior li

SUBMITTER: Willard FS 

PROVIDER: S-EPMC2655719 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature