Gene expression profiles during early differentiation of mouse embryonic stem cells.

ABSTRACT:

Background

Understanding the mechanisms controlling stem cell differentiation is the key to future advances in tissue and organ regeneration. Embryonic stem (ES) cell differentiation can be triggered by embryoid body (EB) formation, which involves ES cell aggregation in suspension. EB growth in the absence of leukaemia inhibitory factor (LIF) leads EBs to mimic early embryonic development, giving rise to markers representative of endoderm, mesoderm and ectoderm. Here, we have used microarrays to investigate differences in gene expression between 3 undifferentiated ES cell lines, and also between undifferentiated ES cells and Day 1-4 EBs.

Results

An initial array study identified 4 gene expression changes between 3 undifferentiated ES cell lines. Tissue culture conditions for

SUBMITTER: Mansergh FC 

PROVIDER: S-EPMC2656490 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature