Real-time quantification of wild-type contaminants in glyphosate tolerant soybean.

ABSTRACT:

Background

Trait purity is a key factor for the successful utilization of biotech varieties and is currently assessed by analysis of individual seeds or plants. Here we propose a novel PCR-based approach to test trait purity that can be applied to bulk samples. To this aim the insertion site of a transgene is characterized and the corresponding sequence of the wild-type (wt) allele is used as diagnostic target for amplification. As a demonstration, we developed a real-time quantitative PCR method to test purity of glyphosate tolerant (Roundup Ready, RR) soybean.

Results

The soybean wt sequence at the RR locus was characterized and found to be highly conserved among conventional genotypes, thus allowing the detection of possibly any soybean non-trait contaminant. On the other

SUBMITTER: Battistini E 

PROVIDER: S-EPMC2656496 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature