Binding of PFOS to serum albumin and DNA: insight into the molecular toxicity of perfluorochemicals.

ABSTRACT:

Background

Health risk from exposure of perfluorochemicals (PFCs) to wildlife and human has been a subject of great interest for understanding their molecular mechanism of toxicity. Although much work has been done, the toxigenicity of PFCs remains largely unknown. In this work, the non-covalent interactions between perfluorooctane sulfonate (PFOS) and serum albumin (SA) and DNA were investigated under normal physiological conditions, aiming to elucidate the toxigenicity of PFCs.

Results

In equilibrium dialysis assay, the bindings of PFOS to SA correspond to the Langmuir isothermal model with two-step sequence model. The saturation binding number of PFOS was 45 per molecule of SA and 1 per three base-pairs of DNA, respectively. ITC results showed that all the interactions we

SUBMITTER: Zhang X 

PROVIDER: S-EPMC2656506 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature