Heterotrimeric coiled coils with core residue urea side chains.

ABSTRACT: We report several coiled coil heterotrimers with varying core residue buried polar groups, all with T(m) values >43 degrees C. Introduction of new synthetic side chain structures, including some terminating in monosubstituted ureas, diversifies the pool of viable core residue candidates. A study of core charge pairings demonstrates that, unlike dimeric systems, trimeric coiled coils do not tolerate guanidine-guanidine contacts, even in the presence of a compensating carboxylate. Overall, the roster of feasible coiled coil designs is significantly expanded.

SUBMITTER: Diss ML 

PROVIDER: S-EPMC2656609 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature