Biological behaviors and proteomics analysis of hybrid cell line EAhy926 and its parent cell line A549.

ABSTRACT:

Background

It is well established that cancer cells can fuse with endothelial cells to form hybrid cells spontaneously, which facilitates cancer cells traversing the endothelial barrier to form metastases. However, up to now, little is known about the biologic characteristics of hybrid cells. Therefore, we investigate the malignant biologic behaviors and proteins expression of the hybrid cell line EAhy926 with its parent cell line A549.

Methods

Cell counting and flow cytometry assay were carried out to assess cell proliferation. The number of cells attached to the extracellular matrix (Matrigel) was measured by MTT assay for the adhesion ability of cells. Transwell chambers were established for detecting the ability of cell migration and invasion. Tumor xenograft test was ca

SUBMITTER: Lu ZJ 

PROVIDER: S-EPMC2657126 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature