Environment-specific combinatorial cis-regulation in synthetic promoters.

ABSTRACT: When a cell's environment changes, a large transcriptional response often takes place. The exquisite sensitivity and specificity of these responses are controlled in large part by the combinations of cis-regulatory elements that reside in gene promoters and adjacent control regions. Here, we present a study aimed at accurately modeling the relationship between combinations of cis-regulatory elements and the expression levels they drive in different environments. We constructed four libraries of synthetic promoters in yeast, consisting of combinations of transcription factor binding sites and assayed their expression in four different environments. Thermodynamic models relating promoter sequences to their corresponding four expression levels explained at least 56% of the variation in expres

SUBMITTER: Gertz J 

PROVIDER: S-EPMC2657533 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature