Estrogenic GPR30 signalling induces proliferation and migration of breast cancer cells through CTGF.

ABSTRACT: The steroid hormone oestrogen can signal through several receptors and pathways. Although the transcriptional responses mediated by the nuclear oestrogen receptors (ER) have been extensively characterized, the changes in gene expression elicited by signalling through the membrane-associated ER GPR30 have not been studied. We show here for ER-negative human breast cancer cells that the activation of GPR30 signalling by oestrogen or by hydroxytamoxifen (OHT), an ER antagonist but GPR30 agonist, induces a transcription factor network, which resembles that induced by serum in fibroblasts. The most strongly induced gene, CTGF, appears to be a target of these transcription factors. We found that the secreted factor connective tissue growth factor (CTGF) not only contributes to promote proliferat

SUBMITTER: Pandey DP 

PROVIDER: S-EPMC2657575 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature