Expression analysis of the Theileria parva subtelomere-encoded variable secreted protein gene family.

ABSTRACT:

Background

The intracellular protozoan parasite Theileria parva transforms bovine lymphocytes inducing uncontrolled proliferation. Proteins released from the parasite are assumed to contribute to phenotypic changes of the host cell and parasite persistence. With 85 members, genes encoding subtelomeric variable secreted proteins (SVSPs) form the largest gene family in T. parva. The majority of SVSPs contain predicted signal peptides, suggesting secretion into the host cell cytoplasm.

Methodology/principal findings

We analysed SVSP expression in T. parva-transformed cell lines established in vitro by infection of T or B lymphocytes with cloned T. parva parasites. Microarray and quantitative real-time PCR analysis revealed mRNA expression for a wide range of SVSP genes. The pat

SUBMITTER: Schmuckli-Maurer J 

PROVIDER: S-EPMC2657828 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature