Bisulfite-based epityping on pooled genomic DNA provides an accurate estimate of average group DNA methylation.

ABSTRACT:

Background

DNA methylation plays a vital role in normal cellular function, with aberrant methylation signatures being implicated in a growing number of human pathologies and complex human traits. Methods based on the modification of genomic DNA with sodium bisulfite are considered the 'gold-standard' for DNA methylation profiling on genomic DNA; however, they require relatively large amounts of DNA and may be prohibitively expensive when used on the large sample sizes necessary to detect small effects. We propose that a high-throughput DNA pooling approach will facilitate the use of emerging methylomic profiling techniques in large samples.

Results

Compared with data generated from 89 individual samples, our analysis of 205 CpG sites spanning nine independent regions of the

SUBMITTER: Docherty SJ 

PROVIDER: S-EPMC2657899 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature