The role of Lhca complexes in the supramolecular organization of higher plant photosystem I.

ABSTRACT: In this work, Photosystem I supercomplexes have been purified from Lhca-deficient lines of Arabidopsis thaliana using a mild detergent treatment that does not induce loss of outer antennas. The complexes have been studied by integrating biochemical analysis with electron microscopy. This allows the direct correlation of changes in protein content with changes in supramolecular structure of Photosystem I to get information about the position of the individual Lhca subunits, the association of the antenna to the core, and the influence of the individual subunits on the stability of the system. Photosystem I complexes with only two or three antenna complexes were purified, showing that the binding of Lhca1/4 and Lhca2/3 dimers to the core is not interdependent, although weak binding of Lhca2/

SUBMITTER: Wientjes E 

PROVIDER: S-EPMC2658074 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature