Chromatin state marks cell-type- and gender-specific replication of the Drosophila genome.

ABSTRACT: Duplication of eukaryotic genomes during S phase is coordinated in space and time. In order to identify zones of initiation and cell-type- as well as gender-specific plasticity of DNA replication, we profiled replication timing, histone acetylation, and transcription throughout the Drosophila genome. We observed two waves of replication initiation with many distinct zones firing in early-S phase and multiple, less defined peaks at the end of S phase, suggesting that initiation becomes more promiscuous in late-S phase. A comparison of different cell types revealed widespread plasticity of replication timing on autosomes. Most occur in large regions, but only half coincide with local differences in transcription. In contrast to confined autosomal differences, a global shift in replication ti

SUBMITTER: Schwaiger M 

PROVIDER: S-EPMC2658520 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature