Micropatterned co-cultures of T-lymphocytes and epithelial cells as a model of mucosal immune system.

ABSTRACT: Gut-associated lymphoid tissue is a major target and reservoir of human immunodeficiency virus (HIV)-infected T-cells. Our studies seek to recapitulate, in vitro, interactions between HIV-infected T-lymphocytes and intestinal epithelial cells in order to investigate the mechanisms underlying the disruption of normal epithelial cell and barrier function. Here, we describe a novel approach for creating co-cultures of healthy or HIV-infected T-lymphocytes (Jurkat) and human intestinal epithelial (HT-29) cells where both cell types are positioned on the same surface in a price spatial configuration (micropattern). This co-culture method simplified observation/monitoring of the two cell types and was particularly suited for laser microdissection-based retrieval of the desired cells for downstre

SUBMITTER: Stybayeva G 

PROVIDER: S-EPMC2659419 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature