A new splice variant of the mouse SIRT3 gene encodes the mitochondrial precursor protein.

ABSTRACT:

Background

Mammals have seven NAD-dependent protein deacetylases. These proteins, called sirtuins, are homologous to yeast Sir2, and are emerging as important regulators of lifespan and intermediary metabolism. Three mammalian sirtuins, SIRT3-5 are mitochondrial. Sirtuins are highly conserved between species, yet mouse SIRT3 was reported to be markedly shorter than its human counterpart and to lack the N-terminal mitochondrial targeting signal present in the human protein.

Results

We have isolated a novel mouse SIRT3 splice variant. This cDNA contains two translation initiation codons upstream of the originally reported start site. We show, using immunofluorescence and protein expression analysis that these longer variants are expressed and efficiently targeted to mitochondr

SUBMITTER: Cooper HM 

PROVIDER: S-EPMC2659428 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature