Local ATP generation by brain-type creatine kinase (CK-B) facilitates cell motility.

ABSTRACT:

Background

Creatine Kinases (CK) catalyze the reversible transfer of high-energy phosphate groups between ATP and phosphocreatine, thereby playing a storage and distribution role in cellular energetics. Brain-type CK (CK-B) deficiency is coupled to loss of function in neural cell circuits, altered bone-remodeling by osteoclasts and complement-mediated phagocytotic activity of macrophages, processes sharing dependency on actomyosin dynamics.

Methodology/principal findings

Here, we provide evidence for direct coupling between CK-B and actomyosin activities in cortical microdomains of astrocytes and fibroblasts during spreading and migration. CK-B transiently accumulates in membrane ruffles and ablation of CK-B activity affects spreading and migration performance. Complementati

SUBMITTER: Kuiper JW 

PROVIDER: S-EPMC2659440 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature