Quantitative analysis of brain nuclear phosphoproteins identifies developmentally regulated phosphorylation events.

ABSTRACT: Protein phosphorylation is a globally adopted and tightly controlled post-translational modification, and represents one of the most important molecular switching mechanisms that govern the entire spectrum of biological processes. In the central nervous system, it has been demonstrated that phosphorylation of key proteins mediating chromatin remodeling and gene transcription plays an important role controlling brain development, synaptogenesis, learning and memory. Many studies have focused on large scale identification of phosphopeptides in brain tissue. These studies have identified phosphorylation site specific motifs useful for predicting protein kinase substrates. In this study, we applied a previously developed quantitative approach, stable isotope labeling of amino acids in mammals

SUBMITTER: Liao L 

PROVIDER: S-EPMC2659596 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature