Genetic diversity of msp3alpha and msp1_b5 markers of Plasmodium vivax in French Guiana.

ABSTRACT:

Background

Reliable molecular typing tools are required for a better understanding of the molecular epidemiology of Plasmodium vivax. The genes msp3a and msp1_block5 are highly polymorphic and have been used as markers in many P. vivax population studies. These markers were used to assess the genetic diversity of P. vivax strains from French Guiana (South America) and to develop a molecular typing protocol.

Methods

A total of 120 blood samples from 109 patients (including 10 patients suffered from more than one malaria episode, samples were collected during each episode) with P. vivax infection were genotyped. All samples were analysed by msp3a PCR-RFLP and msp1_b5 gene sequencing was performed on 57 samples. Genotyping protocol applied to distinguish between new infection o

SUBMITTER: Veron V 

PROVIDER: S-EPMC2660359 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature