Non-destructive sampling of ancient insect DNA.

ABSTRACT:

Background

A major challenge for ancient DNA (aDNA) studies on insect remains is that sampling procedures involve at least partial destruction of the specimens. A recent extraction protocol reveals the possibility of obtaining DNA from past insect remains without causing visual morphological damage. We test the applicability of this protocol on historic museum beetle specimens dating back to AD 1820 and on ancient beetle chitin remains from permafrost (permanently frozen soil) dating back more than 47,000 years. Finally, we test the possibility of obtaining ancient insect DNA directly from non-frozen sediments deposited 3280-1800 years ago -- an alternative approach that also does not involve destruction of valuable material.

Methodology/principal findings

The success of the

SUBMITTER: Thomsen PF 

PROVIDER: S-EPMC2660418 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature