Kruppel-like factor 15, a zinc-finger transcriptional regulator, represses the rhodopsin and interphotoreceptor retinoid-binding protein promoters.

ABSTRACT:

Purpose

To identify novel transcriptional regulators of rhodopsin expression as a model for understanding photoreceptor-specific gene regulation.

Methods

A bovine retinal cDNA library was screened in a yeast one-hybrid assay, with a 29-bp bovine rhodopsin promoter fragment as bait. Expression studies used RT-PCR and beta-galactosidase (LacZ) histochemistry of retinas from transgenic mice heterozygous for a targeted LacZ replacement of KLF15. Promoter transactivation assays measured luciferase expression in HEK293 cells transiently transfected with bovine rhodopsin or IRBP promoter-reporter constructs and expression constructs containing cDNAs for full or truncated KLF15, Crx (cone rod homeobox), and/or Nrl (neural retina leucine zipper). Data were analyzed with general linea

SUBMITTER: Otteson DC 

PROVIDER: S-EPMC2660604 | biostudies-literature | 2004 Aug

REPOSITORIES: biostudies-literature