Promoter methylation correlates with reduced NDRG2 expression in advanced colon tumour.

ABSTRACT:

Background

Aberrant DNA methylation of CpG islands of cancer-related genes is among the earliest and most frequent alterations in cancerogenesis and might be of value for either diagnosing cancer or evaluating recurrent disease. This mechanism usually leads to inactivation of tumour-suppressor genes. We have designed the current study to validate our previous microarray data and to identify novel hypermethylated gene promoters.

Methods

The validation assay was performed in a different set of 8 patients with colorectal cancer (CRC) by means quantitative reverse-transcriptase polymerase chain reaction analysis. The differential RNA expression profiles of three CRC cell lines before and after 5-aza-2'-deoxycytidine treatment were compared to identify the hypermethylated genes.

SUBMITTER: Piepoli A 

PROVIDER: S-EPMC2660908 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature