A multiway analysis for identifying high integrity bovine BACs.

ABSTRACT:

Background

In large genomics projects involving many different types of analyses of bacterial artificial chromosomes (BACs), such as fingerprinting, end sequencing (BES) and full BAC sequencing there are many opportunities for the identities of BACs to become confused. However, by comparing the results from the different analyses, inconsistencies can be identified and a set of high integrity BACs preferred for future research can be defined.

Results

The location of each bovine BAC in the BAC fingerprint-based genome map and in the genome assembly were compared based on the reported BESs, and for a smaller number of BACs the full sequence. BACs with consistent positions in all three datasets, or if the full sequence was not available, for both the fingerprint map and BES-base

SUBMITTER: Ratnakumar A 

PROVIDER: S-EPMC2660975 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature