Filtering for increased power for microarray data analysis.

ABSTRACT:

Background

Due to the large number of hypothesis tests performed during the process of routine analysis of microarray data, a multiple testing adjustment is certainly warranted. However, when the number of tests is very large and the proportion of differentially expressed genes is relatively low, the use of a multiple testing adjustment can result in very low power to detect those genes which are truly differentially expressed. Filtering allows for a reduction in the number of tests and a corresponding increase in power. Common filtering methods include filtering by variance, average signal or MAS detection call (for Affymetrix arrays). We study the effects of filtering in combination with the Benjamini-Hochberg method for false discovery rate control and q-value for false discover

SUBMITTER: Hackstadt AJ 

PROVIDER: S-EPMC2661050 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature