Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR.

ABSTRACT:

Background

Considering the broad variation in the expression of housekeeping genes among tissues and experimental situations, studies using quantitative RT-PCR require strict definition of adequate endogenous controls. For glioblastoma, the most common type of tumor in the central nervous system, there was no previous report regarding this issue.

Results

Here we show that amongst seven frequently used housekeeping genes TBP and HPRT1 are adequate references for glioblastoma gene expression analysis. Evaluation of the expression levels of 12 target genes utilizing different endogenous controls revealed that the normalization method applied might introduce errors in the estimation of relative quantities. Genes presenting expression levels which do not significantly differ betw

SUBMITTER: Valente V 

PROVIDER: S-EPMC2661085 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature