New DArT markers for oat provide enhanced map coverage and global germplasm characterization.

ABSTRACT:

Background

Genomic discovery in oat and its application to oat improvement have been hindered by a lack of genetic markers common to different genetic maps, and by the difficulty of conducting whole-genome analysis using high-throughput markers. This study was intended to develop, characterize, and apply a large set of oat genetic markers based on Diversity Array Technology (DArT).

Results

Approximately 19,000 genomic clones were isolated from complexity-reduced genomic representations of pooled DNA samples from 60 oat varieties of global origin. These were screened on three discovery arrays, with more than 2000 polymorphic markers being identified for use in this study, and approximately 2700 potentially polymorphic markers being identified for use in future studies. DNA se

SUBMITTER: Tinker NA 

PROVIDER: S-EPMC2661094 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature