Ultraspecific probes for high throughput HLA typing.

ABSTRACT:

Background

The variations within an individual's HLA (Human Leukocyte Antigen) genes have been linked to many immunological events, e.g. susceptibility to disease, response to vaccines, and the success of blood, tissue, and organ transplants. Although the microarray format has the potential to achieve high-resolution typing, this has yet to be attained due to inefficiencies of current probe design strategies.

Results

We present a novel three-step approach for the design of high-throughput microarray assays for HLA typing. This approach first selects sequences containing the SNPs present in all alleles of the locus of interest and next calculates the number of base changes necessary to convert a candidate probe sequences to the closest subsequence within the set of sequences

SUBMITTER: Feng C 

PROVIDER: S-EPMC2661095 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature