Transcriptional and functional differences in stem cell populations isolated from extraocular and limb muscles.

ABSTRACT: The extraocular muscles (EOMs) are a distinct muscle group that displays an array of unique contractile, structural, and regenerative properties. They also have differential sensitivity to certain diseases and are enigmatically spared in Duchenne muscular dystrophy (DMD). The EOMs are so distinct from other skeletal muscles that the term "allotype" has been coined to highlight EOM group-specific properties. We hypothesized that increased and distinct stem cells may underlie the continual myogenesis noted in EOM. The side population (SP) stem cells were isolated and studied. EOMs had 15x higher SP cell content compared with limb muscles. Expression profiling revealed 348 transcripts that define the EOM-SP transcriptome. Over 92% of transcripts were SP specific, because they were absent in p

SUBMITTER: Pacheco-Pinedo EC 

PROVIDER: S-EPMC2661100 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature