Fascin-1 promoter activity is regulated by CREB and the aryl hydrocarbon receptor in human carcinoma cells.

ABSTRACT:

Background

Fascin is an actin-bundling protein that is absent from most normal epithelia yet is upregulated in multiple forms of human carcinoma, where its expression correlates clinically with a poor prognosis. How fascin-1 transcription is activated in carcinoma cells is largely unknown, although the hypothesis of regulation by beta-catenin signaling has received attention. The question is important because of the clinical significance of fascin expression in human carcinomas.

Methodology/principal findings

Through comparative genomics we made an unbiased analysis of the DNA sequence of the fascin-1 promoter region from six mammalian species. We identified two regions in which highly conserved motifs are concentrated. Luciferase promoter reporter assays for the human fasci

SUBMITTER: Hashimoto Y 

PROVIDER: S-EPMC2661145 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature