Functional characterization of promoter variants of the adiponectin gene complemented by epidemiological data.

ABSTRACT:

Objective

Adiponectin (APM1, ACDC) is an adipocyte-derived protein with downregulated expression in obesity and insulin-resistant states. Several potentially regulatory single nucleotide polymorphisms (SNPs) within the APM1 gene promoter region have been associated with circulating adiponectin levels. None of them have been functionally characterized in adiponectin-expressing cells. Hence, we investigated three SNPs (rs16861194, rs17300539, and rs266729) for their influence on adiponectin promoter activity and their association with circulating adiponectin levels.

Research design and methods

Basal and rosiglitazone-induced promoter activity of different SNP combinations (haplotypes) was analyzed in 3T3-L1 adipocytes using luciferase reporter gene assays and DNA binding studi

SUBMITTER: Laumen H 

PROVIDER: S-EPMC2661577 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature