TCF7L2 regulates late events in insulin secretion from pancreatic islet beta-cells.

ABSTRACT:

Objective

Polymorphisms in the human TCF7L2 gene are associated with reduced insulin secretion and an increased risk of type 2 diabetes. However, the mechanisms by which TCF7L2 affect insulin secretion are still unclear. We define the effects of TCF7L2 expression level on mature beta-cell function and suggest a potential mechanism for its actions.

Research design and methods

TCF7L2 expression in rodent islets and beta-cell lines was altered using RNAi or adenoviral transduction. Beta-cell gene profiles were measured by quantitative real-time PCR and the effects on intracellular signaling and exocytosis by live cell imaging, electron microscopy, and patch clamp electrophysiology.

Results

Reducing TCF7L2 expression levels by RNAi decreased glucose- but not KCl-induced i

SUBMITTER: da Silva Xavier G 

PROVIDER: S-EPMC2661588 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature