Survey of the human pancreatic beta-cell G1/S proteome reveals a potential therapeutic role for cdk-6 and cyclin D1 in enhancing human beta-cell replication and function in vivo.

ABSTRACT:

Objectives

To comprehensively inventory the proteins that control the G1/S cell cycle checkpoint in the human islet and compare them with those in the murine islet, to determine whether these might therapeutically enhance human beta-cell replication, to determine whether human beta-cell replication can be demonstrated in an in vivo model, and to enhance human beta-cell function in vivo.

Research design and methods

Thirty-four G1/S regulatory proteins were examined in human islets. Effects of adenoviruses expressing cdk-6, cdk-4, and cyclin D1 on proliferation in human beta-cells were studied in both in vitro and in vivo models.

Results

Multiple differences between murine and human islets occur, most strikingly the presence of cdk-6 in human beta-cells versus its low a

SUBMITTER: Fiaschi-Taesch N 

PROVIDER: S-EPMC2661601 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature