Cell-type selective chromatin remodeling defines the active subset of FOXA1-bound enhancers.

ABSTRACT: Selective activity of a specific set of enhancers defines tissue-specific gene transcription. The pioneer factor FOXA1 has been shown to induce functional enhancer competency through chromatin openings. We have previously found that FOXA1 is recruited to thousands of regions across the genome of a given cell type. Here, we monitored the chromatin structure at FOXA1 binding sites on a chromosome-wide scale using formaldehyde assisted isolation of regulatory elements (FAIRE). Surprisingly, we find that a significant fraction of FOXA1-bound sites have a relatively closed chromatin conformation linked to a shift of the epigenetic signature toward repressive histone marks. Importantly, these sites are not correlated with gene expression in a given cell type suggesting that FOXA1 is required, bu

SUBMITTER: Eeckhoute J 

PROVIDER: S-EPMC2661808 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature