A stromal pool of TatA promotes Tat-dependent protein transport across the thylakoid membrane.

ABSTRACT: In chloroplasts and bacteria, the Tat (twin-arginine translocation) system is engaged in transporting folded passenger proteins across the thylakoid and cytoplasmic membranes, respectively. To date, three membrane proteins (TatA, TatB, and TatC) have been identified to be essential for Tat-dependent protein translocation in the plant system, whereas soluble factors seem not to be required. In contrast, in the bacterial system, several cytosolic chaperones were described to be involved in Tat transport processes. Therefore, we have examined whether stromal or peripherally associated membrane proteins also play a role in Tat transport across the thylakoid membrane. Analyzing both authentic precursors as well as the chimeric 16/23 protein, which allows us to study each step of the translocati

SUBMITTER: Frielingsdorf S 

PROVIDER: S-EPMC2662211 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature