Infection and genotype remodel the entire soybean transcriptome.

ABSTRACT:

Background

High throughput methods, such as high density oligonucleotide microarray measurements of mRNA levels, are popular and critical to genome scale analysis and systems biology. However understanding the results of these analyses and in particular understanding the very wide range of levels of transcriptional changes observed is still a significant challenge. Many researchers still use an arbitrary cut off such as two-fold in order to identify changes that may be biologically significant. We have used a very large-scale microarray experiment involving 72 biological replicates to analyze the response of soybean plants to infection by the pathogen Phytophthora sojae and to analyze transcriptional modulation as a result of genotypic variation.

Results

With the unprecedent

SUBMITTER: Zhou L 

PROVIDER: S-EPMC2662884 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature