Gene expression profiles distinguish idiopathic pulmonary fibrosis from hypersensitivity pneumonitis.

ABSTRACT:

Rationale

Many of the interstitial lung diseases represent a diagnostic and therapeutic challenge because their clinical and even histologic features are often nonspecific. Likewise, the transcriptional signatures of most of them are unknown.

Objective

To compare the gene expression patterns from patients with idiopathic pulmonary fibrosis (IPF) hypersensitivity pneumonitis (HP), and nonspecific interstitial pneumonia (NSIP) using custom oligonucleotide microarrays.

Methods

We profiled lung biopsies from 15 patients with IPF, 12 with HP, and eight with NSIP. Labeled complementary ribonucleic acid was hybridized to a custom Affymetrix oligonucleotide DNA microarray using standard Affymetrix protocols. The custom array, Hu03, contained 59,619 probe sets representing an

SUBMITTER: Selman M 

PROVIDER: S-EPMC2662988 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature