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ABSTRACT:
SUBMITTER: Kozarewa I
PROVIDER: S-EPMC2664327 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Kozarewa Iwanka I Ning Zemin Z Quail Michael A MA Sanders Mandy J MJ Berriman Matthew M Turner Daniel J DJ
Nature methods 20090315 4
Amplification artifacts introduced during library preparation for the Illumina Genome Analyzer increase the likelihood that an appreciable proportion of these sequences will be duplicates and cause an uneven distribution of read coverage across the targeted sequencing regions. As a consequence, these unfavorable features result in difficulties in genome assembly and variation analysis from the short reads, particularly when the sequences are from genomes with base compositions at the extremes of ...[more]