Comparison of proteomic and transcriptomic profiles in the bronchial airway epithelium of current and never smokers.

ABSTRACT:

Background

Although prior studies have demonstrated a smoking-induced field of molecular injury throughout the lung and airway, the impact of smoking on the airway epithelial proteome and its relationship to smoking-related changes in the airway transcriptome are unclear.

Methodology/principal findings

Airway epithelial cells were obtained from never (n = 5) and current (n = 5) smokers by brushing the mainstem bronchus. Proteins were separated by one dimensional polyacrylamide gel electrophoresis (1D-PAGE). After in-gel digestion, tryptic peptides were processed via liquid chromatography/ tandem mass spectrometry (LC-MS/MS) and proteins identified. RNA from the same samples was hybridized to HG-U133A microarrays. Protein detection was compared to RNA expression in the curren

SUBMITTER: Steiling K 

PROVIDER: S-EPMC2664466 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature