Microarray-based characterization of a colony assay used to investigate endothelial progenitor cells and relevance to endothelial function in humans.

ABSTRACT:

Objective

An assay proposed to quantify endothelial progenitor cell (EPC) colonies in humans was investigated to determine the phenotype of recovered cells and their relevance to in vivo endothelial function.

Methods and results

Twelve sedentary subjects participating in a worksite wellness program underwent endothelial flow-mediated dilation (FMD) testing of the brachial artery and blood sampling for EPC colony assay. Microarray-based genotypic characterization of colonies showed surface markers consistent with T lymphocyte phenotype, but not with an EPC (CD34, CD133, VEGFR-2) or endothelial (CD146) phenotype. Gene expression patterns more closely matched T lymphocytes (r=0.87) than endothelial cells (r=0.66) in our microarray database. Flow cytometry of colonies confirmed

SUBMITTER: Desai A 

PROVIDER: S-EPMC2664554 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature