Expression-based discovery of candidate ovule development regulators through transcriptional profiling of ovule mutants.

ABSTRACT:

Background

Arabidopsis ovules comprise four morphologically distinct parts: the nucellus, which contains the embryo sac, two integuments that become the seed coat, and the funiculus that anchors the ovule within the carpel. Analysis of developmental mutants has shown that ovule morphogenesis relies on tightly regulated genetic interactions that can serve as a model for developmental regulation. Redundancy, pleiotropic effects and subtle phenotypes may preclude identification of mutants affecting some processes in screens for phenotypic changes. Expression-based gene discovery can be used access such obscured genes.

Results

Affymetrix microarrays were used for expression-based gene discovery to identify sets of genes expressed in either or both integuments. The genes were ide

SUBMITTER: Skinner DJ 

PROVIDER: S-EPMC2664812 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature