Rates of spontaneous cleavage of glucose, fructose, sucrose, and trehalose in water, and the catalytic proficiencies of invertase and trehalas.

ABSTRACT: Most of the early experimental work on enzyme kinetics was based on the properties of yeast invertase (beta-fructo-furanosidase, EC 3.2.1.26), whose robust activity(1) (together with the availability of a continuous polarimetric assay) enabled Michaelis and Menten to establish the existence of a quantitative relationship between the rate of an enzyme reaction and the concentration of its substrate.(2) To appreciate the proficiency of an enzyme as a catalyst, it is also desirable to have information about the relative rates of the catalyzed and uncatalyzed reactions.(3) Surprisingly, the literature discloses no report of the rate of spontaneous hydrolysis of sucrose (although there have been many studies of the acid-catalyzed reaction).(4) That information would be of special interest in vi

SUBMITTER: Wolfenden R 

PROVIDER: S-EPMC2664835 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature