Copper(II) binding to alpha-synuclein, the Parkinson's protein.

ABSTRACT: Variations in tryptophan fluorescence intensities confirm that copper(II) interacts with alpha-synuclein, a protein implicated in Parkinson's disease. Trp4 fluorescence decay kinetics measured for the F4W protein show that Cu(II) binds tightly (Kd 100 nM) near the N-terminus at pH 7. Work on a F4W/H50S mutant indicates that a histidine imidazole is not a ligand in this high-affinity site.

SUBMITTER: Lee JC 

PROVIDER: S-EPMC2664836 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature