In vivo and in vitro protein ligation by naturally occurring and engineered split DnaE inteins.

ABSTRACT:

Background

Protein trans-splicing by naturally occurring split DnaE inteins is used for protein ligation of foreign peptide fragments. In order to widen biotechnological applications of protein trans-splicing, it is highly desirable to have split inteins with shorter C-terminal fragments, which can be chemically synthesized.

Principal findings

We report the identification of new functional split sites in DnaE inteins from Synechocystis sp. PCC6803 and from Nostoc punctiforme. One of the newly engineered split intein bearing C-terminal 15 residues showed more robust protein trans-splicing activity than naturally occurring split DnaE inteins in a foreign context. During the course of our experiments, we found that protein ligation by protein trans-splicing depended not only on

SUBMITTER: Aranko AS 

PROVIDER: S-EPMC2664965 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature