A library-based method to rapidly analyse chromatin accessibility at multiple genomic regions.

ABSTRACT: Traditional chromatin analysis methods only test one locus at the time or use different templates for each locus, making a standardized analysis of large genomic regions or many co-regulated genes at different loci a difficult task. On the other hand, genome-wide high-resolution mapping of chromatin accessibility employing massive parallel sequencing platforms generates an extensive data set laborious to analyse and is a cost-intensive method, only applicable to the analysis of a limited set of biological samples. To close this gap between the traditional and the high-throughput procedures we have developed a method in which a condition-specific, genome-wide chromatin fragment library is produced and then used for locus-specific DNA fragment analysis. To validate the method, we used, as a

SUBMITTER: Basheer A 

PROVIDER: S-EPMC2665225 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature