An evaluation of custom microarray applications: the oligonucleotide design challenge.

ABSTRACT: The increase in feature resolution and the availability of multipack formats from microarray providers has opened the way to various custom genomic applications. However, oligonucleotide design and selection remains a bottleneck of the microarray workflow. Several tools are available to perform this work, and choosing the best one is not an easy task, nor are the choices obvious. Here we review the oligonucleotide design field to help users make their choice. We have first performed a comparative evaluation of the available solutions based on a set of criteria including: ease of installation, user-friendly access, the number of parameters and settings available. In a second step, we chose to submit two real cases to a selection of programs. Finally, we used a set of tests for the in silico

SUBMITTER: Lemoine S 

PROVIDER: S-EPMC2665234 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature