Alternative pre-mRNA splicing switches modulate gene expression in late erythropoiesis.

ABSTRACT: Differentiating erythroid cells execute a unique gene expression program that insures synthesis of the appropriate proteome at each stage of maturation. Standard expression microarrays provide important insight into erythroid gene expression but cannot detect qualitative changes in transcript structure, mediated by RNA processing, that alter structure and function of encoded proteins. We analyzed stage-specific changes in the late erythroid transcriptome via use of high-resolution microarrays that detect altered expression of individual exons. Ten differentiation-associated changes in erythroblast splicing patterns were identified, including the previously known activation of protein 4.1R exon 16 splicing. Six new alternative splicing switches involving enhanced inclusion of internal casse

SUBMITTER: Yamamoto ML 

PROVIDER: S-EPMC2665901 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature