Significant performance variation among PCR systems in diagnosing congenital toxoplasmosis in Sao Paulo, Brazil: analysis of 467 amniotic fluid samples.

ABSTRACT:

Introduction

Performance variation among PCR systems in detecting Toxoplasma gondii has been extensively reported and associated with target genes, primer composition, amplification parameters, treatment during pregnancy, host genetic susceptibility and genotypes of different parasites according to geographical characteristics.

Patients

A total of 467 amniotic fluid samples from T. gondii IgM- and IgG-positive Brazilian pregnant women being treated for 1 to 6 weeks at the time of amniocentesis (gestational ages of 14 to 25 weeks).

Methods

One nested-B1-PCR and three one-round amplification systems targeted to rDNA, AF146527 and the B1 gene were employed.

Results

Of the 467 samples, 189 (40.47%) were positive for one-round amplifications: 120 (63.49%) for the B1

SUBMITTER: Okay TS 

PROVIDER: S-EPMC2666456 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature