A collection of Ds insertional mutants associated with defects in male gametophyte development and function in Arabidopsis thaliana.

ABSTRACT: Functional analyses of the Arabidopsis genome require analysis of the gametophytic generation, since approximately 10% of the genes are expressed in the male gametophyte and approximately 9% in the female gametophyte. Here we describe the genetic and molecular characterization of 67 Ds insertion lines that show reduced transmission through the male gametophyte. About half of these mutations are male gametophytic-specific mutations, while the others also affect female transmission. Genomic sequences flanking both sides of the Ds element were recovered for 39 lines; for 16 the Ds elements were inserted in or close to coding regions, while 7 were located in intergenic/unannotated regions of the genome. For the remaining 16 lines, chromosomal rearrangements such as translocations or deletions,

SUBMITTER: Boavida LC 

PROVIDER: S-EPMC2666506 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature